Termosredsan (solution, medium for thawing cryopreserved bull semen)
Obstetric and gynecological
1 ml of the drug contains:
albumin – 0.55 mg
globulin – 0.36 mg
lactose – 27.5 mg
sodium citrate – 22 mg
L-histidine – 0.55 mg
Description
The liquid is light yellow, transparent.
Pharmacological properties
The components of the drug create osmotic and oncotic pressure in thawed semen, form a source of energy for sperm, high buffering and antibacterial action.
Method of application and dosage
The ampoule with a dose of the drug (1 ml) is opened and placed in a water bath at a temperature of + 38-42 ºC for 2-3 minutes. When the ampoule is already heated, a canister with sperm doses stored in liquid nitrogen is lifted from the Dewar vessel. With sterile and pre-cooled in liquid nitrogen tweezers, the granule is removed from the canister, placed in an ampoule with a warm preparation and thawed for 8-10 seconds. After that, the ampoule is removed from the water bath, wiped with a gauze napkin and placed in a tripod. In preparation for the simultaneous use of two spermatozoa of one bull to accelerate the assessment of sperm motility, it is advisable to merge the contents of two ampoules into one sample and place two granules in them simultaneously.
To improve the preservation of the functional integrity of frozen-thawed sperm in the nutrient cryoprotective medium, which is used to dilute the native sperm of bulls, the drug is applied at a rate of 1:50.
To check the quality of sperm, you should use heated slides and coverslips, which are during the study on the heating table of the microscope or in a box-thermostat. Before taking a drop of semen, the contents of the ampoule are mixed with rotational movements, then a semen is taken with a sterile pipette (or glass rod), placed on a glass slide and covered with a cover.
You can use bull semen, which has the motility of sperm with a rectilinear motion of not less than 4 points (40%).
If deconserved sperm are stored at room temperature and have not been used for 3 hours, their evaluation should be repeated.
Reservation
When working with the drug, the sanitary-hygienic and technological requirements provided by the current instructions on artificial insemination of farm animals should be observed.
Indication
The drug is used in vitro to thaw cryopreserved bull semen, which is stored in the form of unlined granules, in the process of preparing sperm doses for artificial insemination of cows and heifers and (or) adding it to the nutrient cryoprotective medium to dilute fresh bull semen.
Contraindication
None.
Storage conditions
In a dry, dark place at a temperature of 0 to +20 ° C.
Expiration date
1 year (when stored at room temperature), 2 years (when stored in a household refrigerator).